Narrowing Substrate Specificity in a Directly Evolved Enzyme: The A293D Mutant of Aspartate Aminotransferase †,‡

Studies on a Tyr residue critical for th

Studies on a Tyr residue critical for the binding of coenzyme and substrate in mouse 3(17)α-hydroxysteroid dehydrogenase (AKR1C21): structure of the Y224D mutant enzyme

✍ Dhagat, Urmi ;Endo, Satoshi ;Mamiya, Hiroaki ;Hara, Akira ;El-Kabbani, Ossama 📂 Article 📅 2010 🏛 International Union of Crystallography 🌐 English ⚖ 769 KB

Mouse 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) is the only aldo–keto reductase that catalyzes the stereospecific reduction of 3- and 17-ketosteroids to the corresponding 3(17)α-hydroxysteroids. The Y224D mutation of AKR1C21 reduced the__K__~m~value for NADP(H) by up to 80-fold and completely re