Narrow-band fractionation of proteins from whole cell lysates using isoelectric membrane focusing and nonporous reversed-phase separations

Rapid identification and screening of pr

Rapid identification and screening of proteins from whole cell lysates of human erythroleukemia cells in the liquid phase, using non-porous reversed phase high-performance liquid chromatography separations of proteins followed by multi-assisted laser desorption/ionization mass spectrometry analysis and sequence database searching

✍ Yajuan Chen; Dan Wall; David M. Lubman 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 97 KB 👁 2 views

Non-porous reversed phase (NPRP) high-performance liquid chromatography (HPLC) has been used as a rapid method to separate proteins from whole cell lysates of human erythroleukemia (HEL) cells. Using phosphate-buffered saline (PBS) as a lysis buffer to extract proteins from HEL cells, more than 100