Narrow (5-50-μm i.d.) open tubular columns in liquid chromatography using immobilized polymethyloctadecylsiloxane as stationary phase
✍ Scribed by Karin Göhlin; Marita Larsson
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 741 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1040-7685
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✦ Synopsis
Abstract
This paper presents in detail the preparation of highly efficient, stable open tubular (OT) columns for reversed‐phase liquid chromatography. Chromatographic properties of 5‐50‐μm i.d. fused silica columns (1.5‐5 m in length) are described, and details of the coating procedure are discussed. On‐column fluorescence detection was used for the model substituted‐anthracene test solutes. Gas chromatography was used as a complementary technique for column characterization.
The stationary phase studied, immobilized polymethyloctadecylsiloxane, PMSC~18~, was chosen because of its more lipophilic character compared to other siloxanes. As expected, a higher retention power was found compared to polydimethylsiloxane coatings.
The polymeric stationary phase showed good properties. More than 900,000 plates were achieved in 25 min (k = 0.1) in a 5‐μm i.d. fused silica OT column. The stationary phase contribution to band broadening was less than 10% in an 11‐μm i.d. column at a flow rate thirty times u~opt~ (k = 1.3).