Nanopore Technology: Methods and Protocols

✍ Scribed by Monifa A.V. Fahie

• Publisher: Springer US;Humana
• Year: 2021
• Tongue: English
• Leaves: 231
• Series: Methods in Molecular Biology 2186
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This detailed collection explores techniques involved in the main strategies of nanopore sensing, such as translocation, analyte trapping, and interactions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to various biological nanopores, nanopore engineering, and their uses in single molecule sensing, computational methods to study intrinsic nanopore behavior, characterizing the specific translocation activity of a vesicle particle through a nanopore, as well as the use of the technique droplet interface bilayer (DIB) in nanopore and membrane biophysical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Nanopore Technology: Methods and Protocols, with its focus on nanopore technology and biomolecule characterization, will hold the interest of the biophysicists, biochemists, bioengineers, and molecular biologists who are working toward further understanding this key field of research.

✦ Table of Contents

Front Matter ....Pages i-x
Front Matter ....Pages 1-1
Preparation of Fragaceatoxin C (FraC) Nanopores (Natalie Lisa Mutter, Gang Huang, Nieck Jordy van der Heide, Florian Leonardus Rudolfus Lucas, Nicole Stéphanie Galenkamp, Giovanni Maglia et al.)....Pages 3-10
Preparation of Cytolysin A (ClyA) Nanopores (Nicole Stéphanie Galenkamp, Veerle Van Meervelt, Natalie Lisa Mutter, Nieck Jordy van der Heide, Carsten Wloka, Giovanni Maglia)....Pages 11-18
Building Synthetic Transmembrane Peptide Pores (Kozhinjampara R. Mahendran)....Pages 19-32
Design and Assembly of Membrane-Spanning DNA Nanopores (Kerstin Göpfrich, Alexander Ohmann, Ulrich F. Keyser)....Pages 33-48
Front Matter ....Pages 49-49
Determining the Orientation of Porins in Planar Lipid Bilayers (Sandra A. Ionescu, Sejeong Lee, Hagan Bayley)....Pages 51-62
Revelation of Function and Inhibition of Wza Through Single-Channel Studies (Lingbing Kong)....Pages 63-76
Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore (Monifa A. V. Fahie, Bib Yang, Christina M. Chisholm, Min Chen)....Pages 77-94
Nanopore Enzymology to Study Protein Kinases and Their Inhibition by Small Molecules (Leon Harrington, Leila T. Alexander, Stefan Knapp, Hagan Bayley)....Pages 95-114
A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore (Monifa A. V. Fahie, Bach Pham, Fanjun Li, Min Chen)....Pages 115-133
Oligonucleotide-Directed Protein Threading Through a Rigid Nanopore (Garbiñe Celaya, David Rodriguez-Larrea)....Pages 135-144
Unfolding and Translocation of Proteins Through an Alpha-Hemolysin Nanopore by ClpXP (Jeff Nivala, Logan Mulroney, Qing Luan, Robin Abu-Shumays, Mark Akeson)....Pages 145-155
Front Matter ....Pages 157-157
Simulation of pH-Dependent, Loop-Based Membrane Protein Gating Using Pretzel (Alan Perez-Rathke, Monifa A. V. Fahie, Christina M. Chisholm, Min Chen, Jie Liang)....Pages 159-169
Free Energy Minimization for Vesicle Translocation Through a Narrow Pore (Hamid R. Shojaei, Ahad Khaleghi Ardabili, Murrugappan Muthukumar)....Pages 171-183
Front Matter ....Pages 185-185
Single Ion-Channel Analysis in Droplet Interface Bilayer (Arash Manafirad)....Pages 187-195
Continuous and Rapid Solution Exchange in a Lipid Bilayer Perfusion System Based on Droplet-Interface Bilayer (En-Hsin Lee)....Pages 197-211
Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip (Xin Li, Min Chen)....Pages 213-225
Back Matter ....Pages 227-231

✦ Subjects

Life Sciences; Protein Science; Biological and Medical Physics, Biophysics; Biotechnology