Nanofibrous Sugar Sticks Electrospun from Glycopolymers for Protein Separation via Molecular Recognition
✍ Scribed by Qian Yang; Jian Wu; Jun-Jie Li; Meng-Xin Hu; Zhi-Kang Xu
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 315 KB
- Volume
- 27
- Category
- Article
- ISSN
- 1022-1336
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✦ Synopsis
Abstract
Summary: Nanofibrous sugar sticks with linear PANCGAMA and cyclic PANCAG glucose pendants were fabricated by electrospinning from acrylonitrile‐based glycopolymers. Field emission scanning electron microscope was used to characterize the morphologies of the nanofibers. Utilizing the specific carbohydrate‐protein interaction, these two kinds of nanofiber mats were applied to separate the protein mixtures. Con A and BSA were used as model proteins. The Con A/BSA mixture solution was isolated successfully by selective adsorption of Con A to the cyclic glucose pendants on the PANCAG nanofibrous sugar sticks. However, PANCGAMA nanofibers showed almost no selectivity for these two proteins due to the poor specificity between linear glucose pendants and Con A.
Typical SEM images of nanofibers fabricated from PANCAG1.
magnified imageTypical SEM images of nanofibers fabricated from PANCAG1.