Nano-Encapsulation of Lipase by Self-Assembled Nanogels: Induction of High Enzyme Activity and Thermal Stabilization
✍ Scribed by Shin-ichi Sawada; Kazunari Akiyoshi
- Book ID
- 102474291
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 457 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1616-5187
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✦ Synopsis
Abstract
The effects of self‐assembled polysaccharide nanogels on colloidal and thermal stability of lipase from Pseudomonas cepacia were investigated. The enzyme activity, especially k~cat~, drastically increased in the presence of nanogels of cholesterol‐bearing pullulan (CHP). The thermostability of lipase complex increased because the denaturation temperature of lipase increased by more than 20 °C by complexation with CHP nanogels. Lipase denaturation and aggregation upon heating was effectively prevented by complexation with CHP nanogels. Moreover, complexation with CHP nanogels protected lipase from lyophilization‐induced aggregation. Nano‐encapsulation with CHP nanogel is a useful method for colloidal and thermal stabilization of unstable enzyme.
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