Nano-C60: A Novel, Effective, Fluorescent Sensing Platform for Biomolecular Detection

✍ Scribed by Hailong Li; Yingwei Zhang; Yonglan Luo; Xuping Sun

• Book ID: 104593774
• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 802 KB
• Volume: 7
• Category: Article
• ISSN: 1613-6810
• DOI: 10.1002/smll.201100068

✦ Synopsis

Abstract

Water‐soluble nano‐C~60~ can serve as a novel, effective, fluorescent sensing platform for biomolecular detection with high sensitivity and selectivity. In this paper, fluorescent detection of DNA and thrombin via nano‐C~60~ is demonstrated for the first time. The principle of the assay lies in the fact that the adsorption of the fluorescently labeled single‐stranded DNA (ssDNA) probe by nano‐C~60~ leads to substantial fluorescence quenching. In the presence of a target, the biomolecular mutual interaction suppresses this quenching, signaling the existence of the target. This sensing system rivals graphene oxide but is superior to other carbon‐structure‐based systems. The present method can also achieve multiplex DNA detection and withstand the interference from human blood serum.