Na+K+-ATPase activity of different types of striated muscles
โ Scribed by C.David Ianuzzo; Barbara Dabrowski
- Book ID
- 113382579
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 260 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0885-4505
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๐ SIMILAR VOLUMES
There are few published measures of Na ุ ,K ุ -AT-Pase activity in human skeletal muscle. This study investigated the suitability of the K ุ -stimulated 3-Omethylfluorescein phosphatase assay for measurement of Na ุ ,K ุ -ATPase activity in human skeletal muscle. Factors investigated include enzyme
Phalloidin was shown to increase the ATPase activity and Ca z+ sensitivity of both bovine cardiac and rabbit psoas myofibrils when assayed in a solution containing 50 mM KCI, 100 mlvl MOPS (pH 7.0), 2 raM MgClz, I mM ATP, 2 mM EGTA, and varying concentrations of Ca 2รท (temperature 21-22 ยฐ C). The ph