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NADP-specific glutamate dehydrogenase of Penicillium chrysogenum has a homohexamer structure

✍ Scribed by Magdolna Sz. Bogáti; István Pócsi; Judit Maticsek; Péter Boross; József Tózsér; Attila Szentirmai

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 513 KB
Volume: 36
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.3620360512

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✦ Synopsis

The NADP-specific glutamate dehydrogenase of a high B-lactam producing industrial strain of Penicillium chrysogenum was purified to homogeneity. The enzyme (M, = 339000 f 34000) was demonstrated to have a homohexamer quaternary structure with a subunit molecular mass of M, = 56000 f 2000. The N-terminal sequence of the enzyme was also determined and was found to be highly homologous to other fungal NADP-specific glutamate dehydrogenase sequences.

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