NADH in situ laser fluorimetry: Effect of pentobarbital on continuously monitored myocardial redox state
✍ Scribed by Dr. Guy Renault; Martine Muffat-Joly; Juliette Polianski; Roger Ian Hardy; Jean-Louis Boutineau; Jean-Louis Duvent; Jean-Jacques Pocidalo PhD
- Book ID
- 102933018
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 731 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0196-8092
No coin nor oath required. For personal study only.
✦ Synopsis
NAHD laser fluorimetry was applied to in situ monitoring of pentobarbital overload effect on myocardial redox state in rats. Experiments were carried out on open-chest rats anesthetized with pentobarbital. Use of very thin optical probes allowed measurements of NADH fluorescence directly within the myocardial wall. Quantitative analysis of fluorescent and backscattered light signals was made possible on-line.
In 8 animals, a slow IV infusion of 6% pentobarbital (2 mg-mid' for 10 min) induced an average twofold increase in NADH fluorescence, with constant return to baseline level prior to injection after a maximum period of 50 min. Thus, pentobarbital overload strongly affects the myocardial energetic state, and this effect was precisely evaluated with NADH fluorescence monitoring. In situmonitored myocardial redox state variations during pentobarbital infusion can Zonstitute a reference for similar studies on pharmacological agents aimed at protecting tissues against ischemic biochemical damages.