Na+-dependent, active nucleoside transport in S49 mouse lymphoma cells and loss in AE-1 mutant deficient in facilitated nucleoside transport

✍ Scribed by Peter G. W. Plagemann

• Book ID: 102878577
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 521 KB
• Volume: 46
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240460109

✦ Synopsis

S49 rnurine lymphoma cells were examined for expression of various nucleoside transport systems using a non-metabolized nucleoside, forrnycin B, as substrate. Nitrobenzylthioinosine (NBTI)-sensitive, facilitated transport was the primary nucleoside transport system of the cells. The cells also expressed very low levels of NBTI-resistant, facilitated nucleoside transport as well as of Na+-dependent, concentrative formycin B transport. Concentrative transport was specific for uridine and purine nucleosides, just as the concentrative nucleoside transporters of other mouse and rat cells. A nucleoside transport mutant of S49 cells, A€-1, lacked both the NBTI-sensitive, facilitated and Na+-dependent, concentrative forrnycin B transport activity, but Na+-dependent, concentrative transport of a-arninoisobutyrate was not affected.