The tetrameric structure of streptavidin and its exceptionally strong affinity t o biotin (K, = IOl5M-') can be exploited t o achieve an amplification of the signal in immunoassays. In the approach described here streptavidin (STAV) labelled with aminobutylethyl-isoluminol (ABEI) served as a univers
✦ LIBER ✦
N-(β-Carboxypropionyl)luminol as a new chemiluminescence label in immunoassay
✍ Scribed by Hui-Sheng Zhuang; Qiong-E Wang; Fan Zhang; Qiao-Jia Huang; Yu-Chai Tang
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 330 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0256-7660
No coin nor oath required. For personal study only.
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The synthesis of a new acridinium sulphonylamide label for the liquid chromatographic determination of carboxylic acids is described. The label 10-methyl-N-(p-tolyl)-N-(p-iodoacetamidobenzenesulphonyl)-9-acridinium carboxamide iodide is synthesized from 9-acridinecarboxylic acid by a seven-step reac