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N-Methyl-N′-nitro-N-nitrosoguanidine sensitivity of E. coli mutants deficient in DNA methylation and mismatch repair

✍ Scribed by Jones, Mady ;Wagner, Robert


Publisher
Springer
Year
1981
Tongue
English
Weight
180 KB
Volume
184
Category
Article
ISSN
0026-8925

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N-methyl-N'-nitro-N-nitrosoguanidine (NG) induces certain classes of multiple mutations in yeast at high frequency. By selecting for mutation at one locus (his4 or leu1) one frequently obtains double mutants where another mutation to temperature sensitivity has also been induced. This multiple mutag