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N- and C-terminal residues of eIF1A have opposing effects on the fidelity of start codon selection

✍ Scribed by Fekete, Christie A; Mitchell, Sarah F; Cherkasova, Vera A; Applefield, Drew; Algire, Mikkel A; Maag, David; Saini, Adesh K; Lorsch, Jon R; Hinnebusch, Alan G

Book ID
110031989
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
391 KB
Volume
26
Category
Article
ISSN
0261-4189

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## Abstract Transmembrane domains (TMDs) of G‐protein coupled receptors (GPCRs) have very low water solubility and often aggregate during purification and biophysical investigations. To circumvent this problem many laboratories add oligolysines to the __N__‐ and __C__‐termini of peptides that corre