N-Acyl homoserine lactonase promotes prevention of Erwinia virulence with Zwittermicin A-producing strain Bacillus cereus

✍ Scribed by Changming Zhao; Hui Zeng; Ziniu Yu; Ming Sun

• Book ID: 101727893
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 232 KB
• Volume: 100
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.21794

✦ Synopsis

Abstract

Zwittermicin A (ZwA), a hybrid polyketide‐ peptide antibiotic, and N‐acyl ‐homoserine‐lactonase (AHL‐lactonase), a kind of quorum‐quenching enzyme, have the potential to prevent the virulence of Erwinia carotovora, which is a major pathogen of soft rot. The purpose of this study was to combine the two agents in one host to improve their prevention efficacy. AHL‐lactonase was over‐expressed in the ZwA‐producing strain Bacillus cereus and derivates were identified to create genetically modified (GM) strains. The comparative results showed that neither ZwA nor AHL‐lactonase impacted the yield of the other; the increased yield of ZwA could promote the prevention efficacy of GM strains; Coexistence of ZwA and AHL‐lactonase in the GM strains had better prevention efficacy than either has separately. It is a potential therapy to provide more effective prevention and withstand gradually increasing pesticide‐resistance by combining the two antibacterial agents. Biotechnol. Bioeng. 2008;100: 599–603. © 2008 Wiley Periodicals, Inc.