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N-Acetylaspartate and N-acetylaspartylglutamate levels in Alzheimer's disease post-mortem brain tissue

โœ Scribed by Dick Jaarsma; Lammy Veenma-van der Duin; Jakob Korf

Book ID
118931962
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
301 KB
Volume
127
Category
Article
ISSN
0022-510X

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Automated precolumn fluorescence labelli
Automated precolumn fluorescence labelling by carbodiimide activation of N-acetylaspartate and N-acetylaspartylglutamate applied to an HPLC brain tissue analysis
โœ Jakob Korf; Lammy Veenma-van der Duin; Venema Kor; Johannes H. Wolf ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 644 KB

An automated method is described to couple carboxyl-containing metabolites to the fluorophore 2-aminoanthracene in aqueous solution (containing 75% methanol) in the presence of N,N-dicyclohexylcarbodiimide. The reaction was optimized for N-acetylaspartate (N-Ac-Asp) and N-acetylaspartylglutamate (N-