Purification and characterization of an
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Prody, Gerry A. ;Greve, L. Carl ;Hedrick, Jerry L.
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Article
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1985
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John Wiley and Sons
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English
⚖ 426 KB
The enzyme N-acetyl-0-D-glucosaminidase was purified from the cortical granules of Xenopus laevis eggs using affinity chromatography, gel filtration, and density gradient centrifugation. The enzyme had a molecular weight of 37,000-40,000 as determined by polyacrylamide gel electrophoresis and densit