N-acetyl-D-galactosamine-specific lectin isolation from soyflour with poly(HPMA-GMA) beads

✍ Scribed by Işık Perçin; Handan Yavuz; Erol Aksöz; Adil Denizli

• Book ID: 101592491
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 226 KB
• Volume: 111
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/app.29054

✦ Synopsis

Abstract

Soybean lectin was purified from seeds of Glycine max L.Merrill SA88. Poly(hydroxypropyl methacrylate‐glycidyl methacrylate) [poly(HPMA‐GMA)] beads were used as an affinity matrix and N‐acetyl‐D‐galactosamine (GalNAc) was used as an affinity ligand. Soybean lectin adsorption with GalNAc attached poly(HPMA‐GMA) beads from soybean lectin solution (in phosphate buffered saline) was 5.0 mg/g. Maximum adsorption capacity for soybean lectin from the soy flour extract was 26.0 mg/g. Elution of soybean lectin from adsorbent was accomplished by 0.5__M__ galactose solution. Purity of soybean lectin was determined by SDS‐PAGE. It was observed that soybean lectin could be repeatedly adsorbed and desorbed with GalNAc‐attached poly(HPMA‐GMA) beads. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2009