N-(9-acridinyl)-bromoacetamide—A powerful reagent for phase-transfer-catalyzed fluorescence labeling of carboxylic acids for liquid chromatography
✍ Scribed by Stig Allenmark; Monika Chelminska-Bertilsson; Richard A. Thompson
- Book ID
- 102985098
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 630 KB
- Volume
- 185
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Phase-transfer-catalyzed esterification of various carboxylic acids has been carried out on a microanalytical scale with a new reagent, N-(9-acridinyl)-bromoacetamide, in aqueous/organic two-phase systems. The procedure gives esters showing very high fluorescence intensity in acid solution. The large Stokes' shift (greater than 120 nm) means that a low background can be achieved even when using a large emission bandwidth. These derivatives show very good chromatographic behavior on reversed-phase liquid chromatographic columns with the use of aqueous acetonitrile, containing 0.2% of phosphoric acid, as the mobile phase. Detection limits (S/N = 2) as low as 10 fmol can be obtained under optimal conditions.