✦ LIBER ✦
N-(1-Naphthyl)ethylenediamine dihydrochloride as a new reagent for nanomole quantification of sugars on thin-layer plates by a mathematical calibration process
✍ Scribed by M. Bounias
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 397 KB
- Volume
- 106
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
N-(1-Naphthyl)ethylenediamine dihydrochloride dissolved in sulfuric acid-methanol is used as a reagent for determination of sugars on thin-layer plates. The quantification process involves a linear expansion giving a straight calibration line, the equation of which is (peak areas)= = p (amount); the parameters (x) and @ ) are correlated to the number of hydroxyl groups of the molecules. The accuracy ranges from 6 to 0.25%, respectively, in the range 0.06 to 80 nmol of spotted quantities.