Myoglobin as a polymerase chain reaction (PCR) inhibitor: A limitation for PCR from skeletal muscle tissue avoided by the use of thermus thermophilus polymerase
✍ Scribed by Laurent Bélec; Jeacute;rôme Authier; Marie-Christine Eliezer-Vanerot; Cécille Piédouillet; Ali Si Mohamed; Romain K. Gherardi
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 255 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0148-639X
No coin nor oath required. For personal study only.
✦ Synopsis
Skeletal muscle tissue contains polymerase chain reaction (PCR) inhibitors that are coextracted by conventional nucleic acid extraction procedures. Myoglobin, a heme-containing molecule, was shown to act as a potent Thermus aquaticus DNA polymerase inhibitor and is likely to be involved in muscle tissue-associated PCR inhibition. The use of Thermus thermophilus DNA polymerase avoids muscle tissue-associated PCR inhi- bition, and should be used in case of small amounts or instability of the targeted nucleic acid.