Myelopoiesis-associated suppressor-cell activity in mice with lewis lung carcinoma tumors: Interferon-γ plus tumor necrosis factor-α synergistically reduce suppressor cell activity
✍ Scribed by M. Rita I. Young; Melvin E. Young; Mark A. Wright
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- French
- Weight
- 731 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Publication of the International Union Against Cancer Publication de I'Union lnternatlonale Contre le Cancer The myelopoietic stimulation which occurs in mice bearing metastatic Lewis lung carcinoma (LLC-C3) tumors is accompanied by immune suppression and the appearance of myelopoiesis-associated immune suppressor cells in the bone marrow and spleen. Low doses of recombinant murine interferon-y (IFN-y) plus recombinant human tumor necrosis factor-a (TNF-a) were used to limit myelopoiesis and, in turn, reduce the presence of myelopoiesis-associated immune suppressor cells in LLC-C3 tumor bearers. Neither IFN-y nor TNF-a alone had any effect in vitro on the growth of myeloid progenitor cells into colonies or on the suppressive activity of bone-marrow cells from LLGC3-bearing mice. However, the combination of low doses of IFN-y and TNF-a synergistically inhibited both the growth of myeloid progenitor cells into colonies and the suppressive activity of bone-marrow cells from tumor-bearers. Similar results were obtained in vivo. When used alone, neither IFN-y nor TNF-a had any effect on myelopoiesis or on suppressor-cell activity. When combined, IFN-y plus TNF-a synergistically suppressed myelopoiesis and the presence of immune suppressive cells both in the bone marrow and in the spleen of tumor bearers. T-lymphocyte blastogenic and NK cytotoxic activities of the tumor-bearers were restored only after treatment with both IFN-y and TNF-a. 3To whom reprint requests should be sent, at the Dept. of Pathology Research (151-Z2), Hines V.A. Hospital,