Myelin basic protein content of aggregating rat brain cell cultures treated with cytokines and/or demyelinating antibody: Effects of macrophage enrichment

✍ Scribed by A. J. Loughlin; M. N. Woodroofe; M. L. Cuzner; P. Honegger; V. Comte; J. -M. Matthieu

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 770 KB
• Volume: 37
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490370512

✦ Synopsis

The demyelinative potential of the cytokines interleukin-la (IL-la), interferon-y (IFN-y), and tumor necrosis factor-a (TNF-a) has been investigated in myelinating aggregate brain cell cultures. Treatment of myelinated cultures with these cytokines resulted in a reduction in myelin basic protein (MBP) content. This effect was additively increased by anti-myelid oligodendrocyte glycoprotein (a-MOG) in the presence of complement. Qualitative immunocytochernistry demonstrated that peritoneal macrophages, added to the fetal telencephalon cell suspensions at the start of the culture period, successfully integrated into aggregate cultures. Supplementing the macrophage component of the cultures in this fashion resulted in increased accumulation of MBP. The effect of IFN-y on MBP content of cultures was not affected by the presence of macrophages in increased numbers.