𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Myelin-associated glycoprotein (MAG) in the CNS of adult shiverer (Shi/Shi) mice

✍ Scribed by Dr. H.J. Sheedlo; G.J. Siegel

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
985 KB
Volume
16
Category
Article
ISSN
0360-4012

No coin nor oath required. For personal study only.

✦ Synopsis

Brain fractions of adult control (+/+) and shiverer (Shi/Shi) mice were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting . Immunostaining with specific antisera against rat brain myelin-associated glycoprotein (MAG) was detected at about the 96-kD region of gels in electrophoresed samples of the total homogenate, low-speed supernatant fraction, and low-and high-speed sedimentable portions of brain from +/+ mice. Reduced immunostaining was observed in the corresponding samples of brain fractions from Shi/Shi mice.

The cerebrum, cerebellum, and medulla of +/+ and ShiiShi mice were examined immunocytochemically for MAG on paraffin-embedded sections. Periaxonal immunostaining for MAG was observed in all the regions and the highest concentrations were in the corpus callosum, in the central cores of cerebellar folia, and in the medulla. Patterns of distribution were similar in +/+ and Shii Shi mice, although the density of immunostaining around individual axons and the number of immunostained axons were significantly reduced in Shi/Shi mice. In addition, the three brain regions of Shi/Shi mice exhibited oligodendrocyte-like cells that contained immunostaining for MAG in the cytoplasm and periphery of their perikarya. This type of immunostained cell was not observed in + / + mice.

In this study, immunoblotting with brain fractions and immunocytochemistry revealed strong evidence for reduced concentrations of MAG in the CNS of Shii Shi mice compared to control mice. In addition, there is immunocytochemical evidence for abnormal accumulation of MAG in perikarya of oligodendrogliallike cells, suggesting the possibility of a transport block for myelin proteins in the shiverer mutant.

📜 SIMILAR VOLUMES

Myelin-associated glycoprotein is not de
Myelin-associated glycoprotein is not detectable in perikaryal myelin of spiral ganglion neurons of adult mice
✍ Rudolf Martini 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 437 KB

## Abstract The expression of the adhesion molecules N‐CAM (neural cell adhesion molecule), L1, myelin‐associated glycoprotein (MAG), and P0 has been investigated by postembedding‐immunoelectron microscopy in spiral ganglia of adult mice in which both axons and neuronal cell bodies are myelinated.

Multiple functions of the myelin-associa
Multiple functions of the myelin-associated glycoprotein MAG (siglec-4a) in formation and maintenance of myelin
✍ Melitta Schachner; Udo Bartsch 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 776 KB

The myelin-associated glycoprotein, a minor component of myelin in the central and peripheral nervous system, has been implicated in the formation and maintenance of myelin. Although the analysis of MAG null mutants confirms this view, the phenotype of this mutant is surprisingly subtle. In the CNS