Genetic transformation of a mycorrhizal
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Victoria Barrett; Robert K. Dixon; Paul A. Lemke
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Article
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1990
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Springer
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English
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Protoplasts of the ectomycorrhizal fungus, Laccaria laccata, were transformed at frequencies of 5-50 transformants/lxg DNA. Transformation was based on positive selection for resistance to hygromycin B (HmB) using the Escherichia coli aminocyclitol phosphotransferase (aph) gene bracketed by an Asper