Mutator tRNAs are Encoded by the Escherichia coli Mutator Genes mutA and mutC: A Novel Pathway for Mutagenesis

G:C → T:A and G:C → C:G transversions ar

G:C → T:A and G:C → C:G transversions are the predominant spontaneous mutations in the Escherichia coli supF gene: an improved lacZ(am) E. coli host designed for assaying pZ189 supF mutational specificity

✍ Akasaka, Susumu ;Takimoto, Koichi ;Yamamoto, Kazuo 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 682 KB

Escherichia coli K12 strain KS40 and plasmid pKY241 were designed for easy screening of supF mutations in plasmid pZ189. KS40 is a nalidixic acid-resistant (gyrA) derivative of MBM7070 (lacZ(am)CA7020). Using in vitro mutagenesis, an amber mutation was introduced into the cloned gyrA structural gene