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Mutations of RNA polymerase II activate key genes of the nucleoside triphosphate biosynthetic pathways

✍ Scribed by Kwapisz, Marta; Wery, Maxime; Després, Daphné; Ghavi-Helm, Yad; Soutourina, Julie; Thuriaux, Pierre; Lacroute, François


Book ID
109849576
Publisher
Nature Publishing Group
Year
2008
Tongue
English
Weight
779 KB
Volume
27
Category
Article
ISSN
0261-4189

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Linker-insertion mutagenesis was used to isolate mutations in the Saccharomyces cerevisiae gene encoding the largest subunit of RNA polymerase II (RPO21, also called RPB1). The mutant rpo21 alleles carried on a plamid were introduced into a haploid yeast strain that conditionally expresses RPO21 fro