Mutations in the right boundary of Saccharomyces cerevisiae centromere 6 lead to nonfunctional or partially functional centromeres
✍ Scribed by Hegemann, Johannes H. ;Pridmore, Raymond David ;Schneider, Rudolph ;Philippsen, Peter
- Book ID
- 104770895
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 868 KB
- Volume
- 205
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
Centromeres most likely consist of DNA (CEN DNA) interacting with specific proteins. In Saccharomyces cerevisiae a clear picture has emerged of a 120 bp sequence that is characteristic of CEN DNA. We have investigated the 25 bp centromere DNA element (CDEIII) that represents the fight part of a CEN DNA. We showed using a series of mutants generated in vitro that the right most triple A of the consensus sequence TGT.T.TG.. TTCCGAA ..... AAA participates in the assembly of a functional centromere and that no further sequences to the right are needed. Distance changes between the centre dyad TTCCGAA and the triple A have two effects: Addition of one base pair leads to a reduction, and addition of two or four base pairs to a loss of centromere function implying a participation of the centre dyad and the triple A region in protein binding. Indeed, a synthetic oligonucleotide of 39 bp containing CDEIII shows specific protein binding.