Mutations in the Lon gene of E. coli K12 phenotypically suppress a mutation in the sigma subunit of RNA polymerase
โ Scribed by Grossman, Alan D.; Burgess, Richard R.; Walter, William; Gross, Carol A.
- Book ID
- 122466554
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 846 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0092-8674
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Amber mutants of Escherichia coli K-12 affected in the structural gene (rpoD) for th a subunit ofRNA polymerase have been obtained from a strain harboring a temperature-sensitive amber suppressor (supF-Ts6) which is active only at low temperatures. These mutants grow normally at low temperature (30
A suppressor mutation of a temperature-sensitive mutant of ribosomal protein L24 (rplX19) was mapped close to the lon gene by genetic analysis and was shown to affect protease LA. The degradation and the synthesis rates of individual ribosomal proteins were determined. Proteins L24, L14, L15 and L27