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Mutations in the Essential Escherichia coli Gene, yqgF, and Their Effects on Transcription

✍ Scribed by Iwamoto, Akira; Osawa, Atsushi; Kawai, Makiko; Honda, Hirofumi; Yoshida, Saori; Furuya, Nobuhisa; Kato, Jun-ichi


Book ID
119967985
Publisher
S. Karger AG
Year
2012
Tongue
English
Weight
509 KB
Volume
22
Category
Article
ISSN
1464-1801

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Amber mutations in Escherichia coli esse
✍ Delcuve, GeneviοΏ½ve ;CabezοΏ½n, Teresa ;Ghysen, Alain ;Herzog, Albert ;Bollen, Alex πŸ“‚ Article πŸ“… 1977 πŸ› Springer 🌐 English βš– 460 KB

A method to obtain amber mutations in ribosomal protein genes is described. tit relies on the P1-mediated localized mutagenesis (Hong and Ames, 1971) and on the fact that the recipient strain contains (a) an efficient but genetically unstable suppressor, (b) a particular thermoinducible lambda proph