Amber mutations in Escherichia coli esse
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Delcuve, GeneviοΏ½ve ;CabezοΏ½n, Teresa ;Ghysen, Alain ;Herzog, Albert ;Bollen, Alex
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Article
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1977
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Springer
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English
β 460 KB
A method to obtain amber mutations in ribosomal protein genes is described. tit relies on the P1-mediated localized mutagenesis (Hong and Ames, 1971) and on the fact that the recipient strain contains (a) an efficient but genetically unstable suppressor, (b) a particular thermoinducible lambda proph