Mutations in four chloroplast loci ofChlamydomonas reinhadtiiaffecting the photosystem I reaction centers

Introduction of a [4Fe-4S (S-cys)4]+1,+2

Introduction of a [4Fe-4S (S-cys)4]+1,+2 iron-sulfur center into a four-α helix protein using design parameters from the domain of the Fx cluster in the Photosystem I reaction center

✍ M. Paul Scott; John Biggins 📂 Article 📅 2008 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 652 KB

We describe the insertion of an iron-sulfur center into a designed four alpha-helix model protein. The model protein was re-engineered by introducing four cysteine ligands required for the coordination of the mulinucleate cluster into positions in the main-chain directly analogous to the domain pred