Two kinds of yeast secretion vectors were constructed by site-directed mutagenesis of the invertase signal sequence and ligation of synthetic oligonucleotides coding appropriate signals. Each has a cloning site for a foreign gene preceded by a sequence encoding either the signal peptide cleavage sit
Mutations Affecting the Signal Sequence Alter Synthesis and Secretion of Yeast Invertase
β Scribed by Daniel Perlman, Patricia Raney and Harlyn O. Halvorson
- Book ID
- 123633097
- Publisher
- National Academy of Sciences
- Year
- 1986
- Tongue
- English
- Weight
- 1011 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/27855
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π SIMILAR VOLUMES
Various amino acid insertions have been introduced into the proximal portion of the signal sequence of secreted yeast invertase. The altered invertase genes have been reintroduced into yeast and monitored for their ability to direct synthesis of secreted invertase in vivo. The insertions should alte
## Abstract ## Background Although hundreds of different signal peptides have now been identified, few studies have examined the factors enabling signal peptides to augment secretion of mature proteins. Signal peptides, located at the Nβterminus of nascent secreted proteins, characteristically hav