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Mutational specificity: Assessment of 1,3-butadiene mutagenicity in the bone marrow of B6C3F1 lacl transgenic mice (Big Blue®): A review of mutational spectrum and LACL mutant frequency after a 5-day 625 PPM 1,3-butadiene exposure

✍ Scribed by Leslie Recio; Kathy G. Meyer; Linda J. Pluta; Owen R. Moss; Christopher J. Saranko

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
547 KB
Volume
28
Category
Article
ISSN
0893-6692

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✦ Synopsis

Carolina 1,3-Butadiene (BD) is a carcinogen that is bioactivated to at least two genotoxic metabolites. In the present article, we review briefly our previous studies on the in vivo mutagenicity and mutational spectra of BD in bone marrow and extend these studies to examine the effect of exposure time (5-day vs. 4week exposure to 625 ppm BD used in previous studies) on the lac/ mutant frequency in the bone marrow. Inhalation exposure to BD at 625 ppm and 1,250 ppm was mutagenic in vivo, inducing an increase in the transgene mutant and mutation fre-quency in the bone marrow. Analysis of the mutational spectrum in BDexposed and air control mice demonstrated that BD exposure induced an increased frequency of mutations at A:T base pairs. There was no difference in the l a d mutant frequency determined in the bone marrow between a shortterm exposure to BD (5 days) and a longer-term exposure (4 weeks). These data taken together demonstrate that inhalation exposure to BD induces in vivo somatic cell mutation.

📜 SIMILAR VOLUMES

Increased frequency of mutations at A:T
Increased frequency of mutations at A:T base pairs in the bone marrow of B6C3F1 lacl transgenic mice exposed to 1, 3-butadiene
✍ Leslie Recio; Kathy G. Meyer 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 676 KB

W e have examined the spectra of mutations in a collection of 7 4 lac1 mutants isolated from the bone marrow of B6C3F1 lac1 transgenic mice exposed to 1,250 ppm 1,3-butadiene (BD). Of the 49 inde pendent mutations analyzed in the present study, 30 of 49 (61 %) were point mutations at G:C base pairs,

System Issues: Mutagenic response of the
System Issues: Mutagenic response of the endogenous hprt gene and lacl transgene in benzo[a]pyrene-treated Big Blue™ B6C3F1 mice
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Big Blue@ (BB) and generic B6C3F1 mice were given one to three i.p. injections of 50 mg/kg benzo[a]pyrene (B[a]P) in DMSO every other day to achieve cumulative doses of 50 to 150 mg/kg. Three weeks after treatment, the mutation frequency at the endogenous hprt gene and lac/ transgene was measured in