Mutational specificity: An efficient laboratory protocol for the sequencing of large numbers of lacl mutants recovered from Big Blue® transgenic animals
✍ Scribed by Heather L. Erfle; David F. Walsh; James Holcroft; Naheed Hague; Johan G. de Boer; Barry W. Glickman
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 372 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
Mutational spectra provide a powerful approach to investigate both the mutagenic potential and the mechanism of action of suspected mutagens and carcinogens. Recently, transgenic techniques have made it possible to generate mutational spectra in animals. Such a spectrum may consist of 5 0 to 200 mutants depending on the nature of the mutations, and many spectra can be generated depending on the design of the experiment. This report describes a practical approach for the processing and sequencing of large numbers of lac/ mutants recovered from Big Blue@ animals.