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Mutational specificities of environmental carcinogens in the lacl gene of Escherichia coli. III: The cyclic nitrosamine N-nitrosopyrrolidine is a complex mutagen

✍ Scribed by Maria Zielenska; Alia Ahmed; Barry W. Glickman

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
467 KB
Volume
3
Category
Article
ISSN
0899-1987

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✦ Synopsis

Abstract

The mutational specificity of the cyclic nitrosamine N‐nitrosopyrrolidine (NPYR) was determined through the DNA sequence characterization of 33 lacl^−d^ mutations of Escherichia coli. Base substitution was the predominant class of mutation induced (91%). The majority of these (64%) occurred at GC base pairs, in accordance with the predicted significance of NPYR‐derived guanine adducts. In addition, this nitrosamine efficiently produced other kinds of base substitution events as 11 of the 33 mutations occurred at AT base pairs. Deletion, frameshift, and duplication events were also recovered. The complexity of the NPYR mutational spectrum appears to be consistent with the suggestion that this compound acts through both direct and indirect mutational pathways.

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