𝔖 Bobbio Scriptorium
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Mutational effect for stability in a conserved region of thermolysin

✍ Scribed by Y. Matsumiya; K. Nishikawa; K. Inouye; M. Kubo


Book ID
111190541
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
334 KB
Volume
40
Category
Article
ISSN
0266-8254

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Mutations on N-terminal region of Taiwan
✍ Yi-Ling Chiou; Shinne-Ren Lin; Long-Sen Chang πŸ“‚ Article πŸ“… 2008 πŸ› John Wiley and Sons 🌐 English βš– 315 KB

## Abstract In the present study, three Taiwan cobra PLA~2~ variants were prepared by adding an extra __N__‐terminal Met, substituting Asn‐1 by Met or deleting the __N__‐terminal heptapeptide. Recombinant PLA~2~ mutants were expressed in __Escherichia coli__ (__E. coli__), and purified to homogenei