The unusual recombinant plasmid pRC19 carrying the N-terminal fragment of the Escherichia coli RNA polymerase rpoB gene was found to specify high level rifampicin resistance of E. coli cells. Sequence analysis of this plasmid revealed one substitution only: transversion G----T, leading to amino acid
✦ LIBER ✦
Mutation position and type of substitution in the β-subunit of the RNA polymerase influence in-vitro activity of rifamycins in rifampicin-resistant Mycobacterium tuberculosis
✍ Scribed by Bodmer, Thomas; Zürcher, Gabriela; Imboden, Paul; Telenti, Amalio
- Book ID
- 126709958
- Publisher
- Oxford University Press
- Year
- 1995
- Tongue
- English
- Weight
- 218 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0305-7453
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The transducing phage 2 dsupM814 and the plasmid rpIK A l L rpo 8 plB1830 containing the wild-type rpoB gene have been constructed and the primary structure °f the gene's central fragment l l l has been established. In contrast with the wild-type, the gene g G C of the rpoB255 mutant, whose primary