✦ LIBER ✦

Mutation identification DNA analysis system (MIDAS) for detection of known mutations

✍ Scribed by Leonard S. Bazar; G. Bruce Collier; Philip G. Vanek; Barbara A. Siles; Yoke W. Kow; Paul W. Doetsch; Richard P. Cunningham; Jack G. Chirikjian

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 174 KB
Volume: 20
Category: Article
ISSN: 0173-0835
DOI: 10.1002/(sici)1522-2683(19990101)20:6<1141::aid-elps1141>3.0.co;2-7

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Analysis of the amplification refractory

Analysis of the amplification refractory mutation allele-specific polymerase chain reaction system for sensitive and specific detection of p53 mutations in DNA

✍ Low, Eva O.; Jones, Alexandra M.; Gibbins, John R.; Walker, D. Murray 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 84 KB 👁 2 views

The sensitivity of the amplification refractory mutation allele-specific polymerase chain reaction system (ARMAS-PCR) to detect known p53 mutations was determined using DNA extracted from two human tumour cell lines collected by cytobrush, as a model for its use in exfoliative cytology. Using DNA ex

Snapback SSCP analysis: Engineered confo

Snapback SSCP analysis: Engineered conformation changes for the rapid typing of known mutations

✍ Steve D. Wilton; Kaite Honeyman; Sue Fletcher; Nigel G. Laing 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 224 KB 👁 1 views

Several approaches may be applied to detect known mutations, including restriction enzyme cleavage, allele-specific oligonucleotide (ASO) hybridization or amplification, dideoxy fingerprinting, and direct DNA sequencing. All these approaches require several extra steps after PCR and may involve radi

Comparative sequence analysis (CSA): A n

Comparative sequence analysis (CSA): A new sequence-based method for the identification and characterization of mutations in DNA

✍ Christopher Mattocks; Patrick Tarpey; Martin Bobrow; Joanne Whittaker 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 385 KB 👁 2 views

Direct sequencing analysis is largely used to confirm and characterize mutations previously detected by more rapid tests. We have developed a method-Comparative Sequence Analysis (CSA)that simplifies the analysis of sequencing data facilitating its use as a first screen for mutation detection. Seque

Detection of known and new mutations in

Detection of known and new mutations in the thiopurine S-methyltransferase gene by single-strand conformation polymorphism analysis

✍ Catherine Spire-Vayron de la Moureyre; Hervé Debuysère; Nadda Sabbagh; Delphine 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 467 KB 👁 2 views

To detect mutations in the thiopurine S-methyltransferase gene (TPMT), we have developed a strategy based on single-strand conformation polymorphism (SSCP) analysis of the gene amplified by polymerase chain reaction (PCR). The sensitivity of the method was first evaluated by analyzing DNA samples fr

Mutational analysis of the hMLH1 gene us

Mutational analysis of the hMLH1 gene using an automated two-dimensional DNA typing system

✍ Shin Sasaki; Takashi Tokino; Takashi Miyatsu; Tetsuichiro Muto; Yusuke Nakamura 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 241 KB 👁 1 views

Searching for mutations in DNA mismatch repair genes is important not only for presymptomatic diagnosis, but also for documenting the spectrum of mutations among families carrying predispositions to hereditary nonpolyposis colorectal cancer (HNPCC). We utilized an automated two-dimensional DNA typin

Identification of three novel mutations

Identification of three novel mutations in the USH1C gene and detection of thirty-one polymorphisms used for haplotype analysis

✍ Ingrid Zwaenepoel; Elisabeth Verpy; Stéphane Blanchard; Moritz Meins; Eckart Apf 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 234 KB 👁 2 views