## Communicated by Jean-Claude Kuplan We developed a parallel denaturing gradient gel electrophoresis (DGGE) protocol to maximize the detection of nucleotide variants in the D N A sequence coding for the mature insulin receptor and in splice site junctions. The melting behaviours of exons 2 to 22
✦ LIBER ✦
Mutation detection of the human glucocorticoid receptor alpha gene area coding for the hormone-binding domain by denaturing gradient gel electrophoresis
✍ Scribed by A.F. Tsolakidou; S.A. Coulocheri; C.E. Sekeris; P. Moutsatsou
- Book ID
- 117331194
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 121 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0165-022X
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We report the set-up of a denaturant gradient gel electrophoresis (DGGE) assay to screen for mutations in the whole coding sequence of the p53 gene. These DGGE experimental conditions were applied to the analysis of the p53 gene in acute leukemias. Forty adults with acute myelogenous leukemia (AML)