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Mutation breeding ofChrysanthemum morifoliumRam.using in vivo and in vitro adventitious bud techniques

✍ Scribed by C. Broertjes; S. Roest; G. S. Bokelmann

Publisher: Springer
Year: 1976
Tongue: English
Weight: 508 KB
Volume: 25
Category: Article
ISSN: 0014-2336
DOI: 10.1007/bf00041524

No coin nor oath required. For personal study only.

✦ Synopsis

Chrysanthemum morifolium, explants, mutation breeding, adventitious bud technique, chimeral and nonchimeral mutants, in vivo, in vitro, X-rays, solid mutants.

SUMMARY

During experiments, which are being carried out to study the factors which control the process of adventitious bud formation in vivo on detached leaves of Chrysanthemum morifolium RAM, adventitious shoots were produced from leaves, irradiated with 500 rad of X-rays. The most important but disadvantageous result was that the majority of the adventitious shoots proved to be of a chimeral nature and obviously developed from more than one cell.

An in vitro adventitious bud technique was developed using different types of explants. Pedicel segments regenerated the highest number of adventitious shoots and, moreover, they developed faster as compared to explants of young flower heads or leaves. The mutants produced by irradiating the various explants were almost exclusively of a solid (non-chimeral) nature. In addition, histological observations suggest that single epidermal cells are involved in the initiation of the adventitious shoot apices.

The optimum dose for mutant production is approximately 800 rad X-rays. Rather often, more than one phenotypically identical mutant was found, which was always derived from the same explant. They could for instance originate from a multi-apical meristem formed by a single mutated cell.

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