Mutants of luminous bacteria selected for bioluminescent toxicity tests

✍ Scribed by Wagner, A. ;Winkler, U. ;Lümmen, P.

• Book ID: 101716082
• Publisher: John Wiley and Sons
• Year: 1989
• Weight: 257 KB
• Volume: 4
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170040146

✦ Synopsis

Mutants of the luminescent bacterial strain NRRL 6-1 1177 were isolated with pleiotropic hypersensitivity towards hydrophobic antimicrobial agents. SDS-PAGE analyses of outer membrane proteins and lipopolysaccharides revealed that the outer membrane structure of the ahs-mutants was altered. QSAR analysis showed that the inhibitory effect of chloro-substituted phenols on bioluminescence of the ahs-mutants depended on their hydrophobicity. The effect of chlorinated phenols and detergents on bioluminescence was increased in the ahs-mutants. The potential use of these mutants in bioluminescent toxicity tests was discussed.