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Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains ofClostridium thermocellum

โœ Scribed by Xiongjun Shao; Babu Raman; Mingjun Zhu; Jonathan R. Mielenz; Steven D. Brown; Adam M. Guss; Lee R. Lynd


Book ID
105953495
Publisher
Springer
Year
2011
Tongue
English
Weight
813 KB
Volume
92
Category
Article
ISSN
1432-0614

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Two Clostridium thermocellum strains were improved for ethanol tolerance, to 5% (v/v), by gradual adaptation and mutation. The best mutant gave an ethanol yield of 0.37 g/g substrate, with a growth yield 1.5 times more than its parent. Accumulation of acids and reducing sugars by the mutant strain w