to the previously established Big Blue ® assay. Genomic DNA isolated from liver, spleen, and lung tissue of control or ethylnitrosourea (ENU)-treated Big Blue ® mice (100 mg/kg i.p., single dose) was packaged into phage (five animals, two packagings per DNA sample) which were simultaneously plated f
Mutant frequencies and mutation spectra of dimethylnitrosamine (DMN) at the lacI and cII loci in the livers of Big Blue® transgenic mice
✍ Scribed by B.S Shane; D.L Smith-Dunn; J.G de Boer; B.W Glickman; M.L Cunningham
- Book ID
- 114070246
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 113 KB
- Volume
- 452
- Category
- Article
- ISSN
- 0027-5107
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Big Blue ® mice harbor a recoverable transgene in a lambda/LIZ shuttle vector. In the standard assay, in vivo mutations are measured in the bacterial lacI gene using a labor-intensive color plaque assay. Applying a simpler assay : Proc Natl Acad Sci USA 93:9073-9078], we measured mutations in the la
We determined the spectrum of mutations in the lacI gene in the liver of Big Blueா transgenic mice after exposure to five daily doses of 2 mg/kg dimethylnitrosamine (DMN) at 3 and 6 weeks of age. This dose has been reported to increase the mutant frequency 9-fold when the animals are 3 weeks old. Th