𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Mutant frequencies and mutation spectra of dimethylnitrosamine (DMN) at the lacI and cII loci in the livers of Big Blue® transgenic mice

✍ Scribed by B.S Shane; D.L Smith-Dunn; J.G de Boer; B.W Glickman; M.L Cunningham


Book ID
114070246
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
113 KB
Volume
452
Category
Article
ISSN
0027-5107

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Comparison of mutant frequencies at the
✍ D.M. Zimmer; P.R. Harbach; W.B. Mattes; C.S. Aaron 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 114 KB 👁 2 views

to the previously established Big Blue ® assay. Genomic DNA isolated from liver, spleen, and lung tissue of control or ethylnitrosourea (ENU)-treated Big Blue ® mice (100 mg/kg i.p., single dose) was packaged into phage (five animals, two packagings per DNA sample) which were simultaneously plated f

Spontaneous mutation spectrum at the lam
✍ P.R. Harbach; D.M. Zimmer; A.L. Filipunas; W.B. Mattes; C.S. Aaron 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 241 KB 👁 2 views

Big Blue ® mice harbor a recoverable transgene in a lambda/LIZ shuttle vector. In the standard assay, in vivo mutations are measured in the bacterial lacI gene using a labor-intensive color plaque assay. Applying a simpler assay : Proc Natl Acad Sci USA 93:9073-9078], we measured mutations in the la

Mutational spectrum of dimethylnitrosami
✍ Johan G. de Boer; Jon C. Mirsalis; Barry W. Glickman 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 49 KB 👁 2 views

We determined the spectrum of mutations in the lacI gene in the liver of Big Blueா transgenic mice after exposure to five daily doses of 2 mg/kg dimethylnitrosamine (DMN) at 3 and 6 weeks of age. This dose has been reported to increase the mutant frequency 9-fold when the animals are 3 weeks old. Th