Mutagenicity of HPLC-fractionated urinary metabolites from 2,4,6-trinitrotoluene-treated Fischer 344 rats
✍ Scribed by L. R. Brooks; R. W. Jacobson; S. H. Warren; M. J. Kohan; K. C. Donnelly; S. E. George
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 63 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0893-6692
No coin nor oath required. For personal study only.
✦ Synopsis
The production and storage of explosives has re-TA98NR, TA100, and TA100NR without metabolic sulted in the environmental accumulation of the mu-activation using a microsuspension modification of tagen 2,4,6-trinitrotoluene (TNT). In order to char-the Salmonella histidine reversion assay. Fractions acterize the production of mutagenic urinary metab-3, 5-18, 21, 22, and 24 -26 contained mutagens olites, 6-week old male Fischer 344 rats were detected by strain TA98. In the nitroreductase-defiadministered 75 mg of TNT/kg or DMSO vehicle cient strain TA98NR, some mutagenic activity was by gavage. The animals were placed into metabo-lost; however, fractions 3, 6, 9-11, 15, and 25 lism cages, and urine was collected for 24 hr. Fol-clearly contained direct-acting mutagens. Fewer lowing filtration, metabolites in the urine were de-fractions were positive in strain TA100 (9-16, 19, conjugated with sulfatase and b-glucuronidase and 20, and 25) with less activity observed in the nitroconcentrated by solid phase extraction. The eluate reductase deficient strain TA100NR (fractions 3, was fractionated by reverse-phase high-perfor-12, 14, 15, and 25). Although some mutagenic mance liquid chromatography (HPLC) using aceto-activity coeluted with known TNT metabolite stannitrile/water, and the fractions were solvent ex-dards, there were still many unidentified mutagenic changed in DMSO by nitrogen evaporation. Each peaks.