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Mutagenesis of the fructose-6-phosphate−binding site in the 2-kinase domain of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

✍ Scribed by Luc Bertrand; Didier Vertommen; Phillip M. Freeman; Johan Wouters; Eric Depiereux; Attilio Di Pietro; Louis Hue; Mark H. Rider

Book ID
114429189
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
581 KB
Volume
254
Category
Article
ISSN
1432-1327

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## Abstract Reversible unfolding of rat testis fructose 6‐phosphate, 2‐kinase:fructose 2,6‐bisphosphatase in guanidine hydrochloride was monitored by following enzyme activities as well as by fluorescence methodologies (intensity, emission maximum, polarization, and quenching), using both intrinsic