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Murine spontaneous T-cell leukemia constitutively expressing IL-2 receptor—a model for human T-cell malignancies expressing IL-2 receptor

✍ Scribed by Hefziba Lugasi; Silvia Hajos; John R. Murphy; Terry B. Strom; Jean Nichols; Carlos Peñarroja; David Naor

Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
577 KB
Volume
45
Category
Article
ISSN
0020-7136

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✦ Synopsis

We describe a new, spontaneously occurring BALBk-derived murine T-cell leukemia. The leukemic cells, designated LB, grow rapidly and progressively in the syngeneic host with no signs of effective immunological resistance. LB cells expressed the Thy-l+, Lyt-2+, UT4-, CD3-class-l+, CD25+ (IL-2 receptor, IL-ZR), class-ll-, gp70-phenotype. As LB cells express IL-2, as indicated by staining with 2 distinct anti-CD25 IL-2R monoclonal antibodies (MAbs), the therapeutic efficacy of IL-2-diphtheria toxin-related protein was tested on this leukemic model. IL-2-diphtheria toxin, but not diphtheria toxin, efficiently inhibited the proliferation of LB cells. The proliferation of a murine myeloma cell line, which does not express IL-2% was not inhibited by IL-2-diphtheria toxin. The possible implantation of this animal model in fundamental and practical studies is discussed.

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