Murine sarcoma virus related nucleic acid sequences in a non-transforming virus derived from an interspecies pseudotype sarcoma virus
✍ Scribed by Hiromi Okabe; Raymond V. Gilden; Masakazu Hatanaka
- Book ID
- 102868501
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- French
- Weight
- 766 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
A number of sarcomagenic viruses recovered from hamster tumors induced originally by Moloney sarcoma virus or several of its mouse pseudotypes appear to be interspecies pseudotypes with structural proteins of hamster type‐C viruses and nucleic acid sequences of both mouse and hamster specificity. A non‐transforming virus (GLOH^−^) recovered from a sarcomagenic virus preparation obtained from a tumor induced by the Gross pseudotype of Moloney sarcoma virus was previously shown to retain mouse sequences. The ability to discriminate readily between Gross and Moloney viruses by molecular hybridization, especially with regard to thermal stability of inter‐strain hybrid molecules, and the stability of Moloney virus sequences in a rat‐mouse interspecies pseudotype, made it possible to specify that the mouse virus‐specific sequences in GLOH^−^ are derived from Moloney virus and not Gross. Thus, even though GLOH^−^ is non‐transforming in vitro or in vivo, it still retains sequences highly related to Moloney sarcoma virus. The lack of transforming ability is thus probably based on loss or change of specific sequences necessary for transformation at some point in the history of this virus.