Murine H-2Dd-reactive monoclonal antibodies recognize shared antigenic determinant(s) on human HLA-B7 or HLA-B27 molecules or both
✍ Scribed by Najet Rebai; Pierre Mercier; Tom Kristensen; Christian Devaux; Bernard Malissen; Claude Mawas; Michel Pierres
- Publisher
- Springer-Verlag
- Year
- 1983
- Tongue
- English
- Weight
- 974 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0093-7711
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✦ Synopsis
We have evaluated the serological relationships between the murine H-2D d and human HLA molecules using four H-2Dd-reactive monoclonal antibodies (mAbs) produced in the A.BY (KblbD b) anti-A.TL (K~IkD d) combination. In the mouse, these reagents exhibited three distinct reactivity patterns: D d, K S, and H-2 u (mAb 81.L); D a, H-2 p, and H-2 u (mAb 81.R); and D d, K d, H-2 p, H-2 u, and H-2 v (mAbs 97.G and 97.H). Sequential immunoprecipitation and cross-competitive mAb binding experiments revealed that these mAbs recognized determinants in two spatially distinct polymorphic domains on the H-2D d molecule of B10.A(5R) cells (defined by mAbs 81.L and 81.R, 97.H, and 97.G, respectively). MAbs 81.R, 97.G, and 97.H, but not 81i, also defined an HLA-linked polymorphism in the human, the main characteristics of which can be summarized as follows: (i) on B lymphoblast6id cell lines, mAbs 81.R and 97.H bound to cells expressing the HLA-B7, HL-B27 or -Bw40 cross-reacting specificities, (ii) on peripheral blood lymphocyte (PBL) panel n~,b 81.R exerted C dependent cytotoxicity to 118 of 400 cells tested, including almost all HLA-B7 or HLA-B27 cells or both (r: 0.952), (iii) the expression of the 81.R cross-reacting determinant segregated in an informative family with the parental haplotype carrying the HLA-B7 allele, and (iv) mAbs 81.R, 97.G, and 97.H recognized topologically related determinants on the same class I molecule(s) of the human B lymphoblastoid ceils JY (HLA-A2,2, -B7,7). These data support the view that some, but not all H-2D d allotopes have been conserved througJhout evolution and are associated in the human with the HLA-B7, -B27 cross-reacting specificities.