Multivariate Optimization of Polymerase Chain Reaction for Microbial Community Analysis
✍ Scribed by Ingela Dahllöf; Staffan Kjelleberg
- Book ID
- 106281496
- Publisher
- Springer
- Year
- 2002
- Tongue
- English
- Weight
- 202 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1053-6426
No coin nor oath required. For personal study only.
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## Abstract An integrated system of a silicon‐based microfabricated polymerase chain reaction (μPCR) chamber and microfabricated electrophoretic glass chips have been developed. The PCR chamber was made of silicon and had aluminum heaters and temperature sensors integrated on the glass anodically b
Aequorin-based flash-type bioluminescent methods can detect nucleic acid molecules in the attomolar range (10(-18)) enabling improved monitoring of the polymerase chain reaction (PCR) at cycles previously considered too low for product detection. The high sensitivity of bioluminescence (BL) was used