Multivariate analysis of 2-DE protein patterns – Practical approaches
✍ Scribed by Susanne Jacobsen; Harald Grove; Kristina Nedenskov Jensen; Helle A. Sørensen; Flemming Jessen; Kristin Hollung; Anne Kjersti Uhlen; Bo M. Jørgensen; Ellen Mosleth Færgestad; Ib Søndergaard
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 458 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0173-0835
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## Abstract We describe the application of a general method of comparing and sorting the patterns of protein expression obtained from two‐dimensional (2‐D) electrophoresis of a mixture of proteins. The method involved the use of two multidimensional classification programs and was applied, first, t
## Abstract 2‐DE is a fundamental technology used in proteomics research. However, despite its high capacity to simultaneously separate several proteins for subsequent identification and quantitative comparison studies, a drawback for this technique is its limited reproducibility, especially when c